aav-mediated transgene delivery of a gcamp6s calcium reporter aav1-hsyn1-mruby2-gsg-p2a-gcamp6s-wpre-pa  (Addgene inc)

Journal: Neuron

Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex

doi: 10.1016/j.neuron.2019.10.007

Figure Lengend Snippet: (A) Schematic representation of the experimental timeline (A1). Experiments were performed in non-anesthetized GAD67-GCaMP6s pups between postnatal days 4 (P4) and 11 (P11). Location of the fields of view (FOV, green lines) at P4-P6 (superficial layers = 13, and deep = 6), P7-P9 (superficial = 17, deep = 7) and P10-P11 (superficial = 7, deep = 5) (A2). Each line represents one imaged field of view.

Article Snippet: Spontaneous Correlated Activity of Diverse GABAergic Components In Vivo To record spontaneous activity of large ensembles of GABAergic neurons in vivo, we generated mice expressing the calcium reporter protein GCaMP6s exclusively in GABA neurons by crossing GAD67Cre mice ( Melzer et al., 2012 ) with the reporter line Ai96 (RCL-GCaMP6s) (The Jackson Laboratory, and ).

Techniques:

Journal: Neuron

Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex

doi: 10.1016/j.neuron.2019.10.007

Figure Lengend Snippet: (A) Schematic representation of the cells (GABAergic neurons) targeted with the GAD67-GCaMP mouse line (A1). Gray triangles represent glutamatergic neurons and green indicates cells expressing GCaMP6s; perisomatic RINGS are large open circles. Confocal images indicating co-labeling (arrows) between GCaMP (green) and synaptotagmin-2 (Syt2, red) at P8 around the somata of putative glutamatergic neurons in the deep (top) and superficial (bottom) layers of the barrel cortex (A2). Scale bar, 20 μm.

Article Snippet: Spontaneous Correlated Activity of Diverse GABAergic Components In Vivo To record spontaneous activity of large ensembles of GABAergic neurons in vivo, we generated mice expressing the calcium reporter protein GCaMP6s exclusively in GABA neurons by crossing GAD67Cre mice ( Melzer et al., 2012 ) with the reporter line Ai96 (RCL-GCaMP6s) (The Jackson Laboratory, and ).

Techniques: Expressing, Labeling

Journal: Neuron

Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex

doi: 10.1016/j.neuron.2019.10.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Spontaneous Correlated Activity of Diverse GABAergic Components In Vivo To record spontaneous activity of large ensembles of GABAergic neurons in vivo, we generated mice expressing the calcium reporter protein GCaMP6s exclusively in GABA neurons by crossing GAD67Cre mice ( Melzer et al., 2012 ) with the reporter line Ai96 (RCL-GCaMP6s) (The Jackson Laboratory, and ).

Techniques: Virus, Software